Regulatory Region of Expression of Thiobacillus ferrooxidans leuB Gene in Escherichia coli

Abstract

The regulatory region of Thiobacillus ferrooxidans leuB gene was analyzed. The deletion analysis indicated that the promoter sequences CATCCG and TATTAT, which were similar to the consensus -35 and -10 sequences of Escherichia coli, respectively, existed. The transcriptional initiation site was located at the position of cytosine -26. The deletion analyrsis of the upstream region suggested the existence of a regulatory region by leucine and the region related to transcription of the gene.