Flutolanil Resistance as a Genetic Marker of Coprinus cinereus Strains

Shigeru OITA; Tsutomu FUSHIMI; Tetsuya OOKURA; Yasuhiro ITO; Sonoe O. YANAGI

doi:10.1271/bbb.61.2145

Shigeru OITA, Tsutomu FUSHIMI, Tetsuya OOKURA, Yasuhiro ITO, Sonoe O. YANAGI

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Keywords: flutolanil, drug resistant marker, Coprinus cinereus, Schizophyllum commune, basidiomycete

JOURNAL FREE ACCESS

1997 Volume 61 Issue 12 Pages 2145-2147

DOI https://doi.org/10.1271/bbb.61.2145

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Abstract

All 4 Schizophyllum commune strains among several basidiomycete species were exclusively resistant to flutolanil, which inhibits succinate dehydrogenase complex (SDC) function. Flutolanil resistant strains could be screened from monokaryotic protoplasts of sensitive Coprinus cinereus, by treating them with a cloned genomic SDC iron-sulfur protein (IP) gene from the resistant S. commune. The obtained resistance was stably transferred to the progeny.

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