Author's Organization：Department of Food Science and Technology, Faculty of Agriculture, Kyoto University Department of Food Science and Technology, Faculty of Agriculture, Kyoto University Department of Food Science and Technology, Faculty of Agriculture, Kyoto University Department of Food Science and Technology, Faculty of Agriculture, Kyoto University:(Present address)Research Institute for Biology-Oriented Science and Technology, Kinki University
A glass microfiber filter was found useful for detection of the complex of lysyl-tRNA synthetase from Bacillus stearothermophilus with lysyladenylate. Detected c.p.m. values of the 3H-labeled complex trapped by the glass microfiber filter was about 2-fold higher than those by nitrocellulose filter and about 3-fold higher than those by DEAE-cellulose filter because of its higher counting efficiency. The use of the glass fiber filter is more suitable for the lysyl-tRNA synthetase assay than the use of either nitrocellulose or DEAE-cellulose filters, which were used for other aminoacyl-tRNA synthetases. Effects of pH on the filter assay of lysyl-tRNA synthetase activity using the glass microfiber filter were also examined.
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