Abstract
The rate parameters for maltooligosaccharides of two α-glucosidases from spinach seeds were examined and subsite affinities were evaluated. The subsite affinities for subsites 1, 2, 3, 4, 5, 6, and 7 in the active site of α-glucosidase A and B were 1.73, 2.91, 1.10, 0.25, 0.94, 0.03, and -0.01 kcal/mol, and 0.33, 4.94, 0.26, 0.14, -0.24, -0.52, and 0.14 kcal/mol, respectively. The six and four subsites exist in α-glucosidase A and B, respectively, which maltooligosaccharides or soluble starch could be bound.
