Bioscience and Microflora
Online ISSN : 1349-8355
Print ISSN : 1342-1441
ISSN-L : 1342-1441
Formation of Glutamine by Combination of Bifidobacterial Sugar Fermentation and Bacterial Glutamine Synthetase
Toshihiro YANOYuuji MISHIMATakashi TACHIKIHidehiko KUMAGAITatsurokuro TOCHIKURA
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1999 年 18 巻 1 号 p. 31-36

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Glutamine formation was observed by coupling the reaction of bacterial glutamine synthetase with the sugar fermentation system of Bifidobacterium pseudolongum c. About 5 mM glutamine was formed in 1 hr of incubation at 37°C with 15 mM glutamate, 15 mM NH4Cl, 30 mM potassium phosphate buffer (pH 7.0), 10 mM glucose, 3.4 mg/ml toluol-treated B. pseudolongum c cells and 50 units/ml glutamine synthetase. The reaction proceeded in autoclaved swine feces without the addition of NH4Cl. In the toluol-treated fresh feces of a breast-fed infant where bifidobacterial growth occurred preferentially, glutamine formation proceeded without the addition of bifidobacterial cells, NH4Cl, or phosphate, however glutamate was necessary. Based on these results, ammonia detoxification in the intestine by bifidobacteria was discussed.

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