抄録
An ion channel in ciliary membrane vesicles isolated from Tetrahymnena pyriformis NT-1 was characterized with a single-channel recording technique applied to the membrane reconstituted into a planar lipid bilayer made of soybean phospholipid. The gating of this channel is independent of membrane potential and it spends most of the time in its open-channel state. It is selective for metal cations over gluconate anion although it little distinguishes cations, Ca2+, Ba2+, Mg2+, and K+. This channel seems to be identical to the monovalent cation channel in Tetrahymena thermophila BII cilia, which was recently reported by Oosawa and Sokabe (Am. J. Physiol. 249, C177-0179, 1985). The channel conductance saturates with increasingion concentration. The maximum value (in pS) for the single-channel conductance obtained in symmetric solutions of sufficiently high concentration is in the order of K+(212) > Ba2+(41) > Ca2+ (22) > Mg2+ (20). On the other hand, the affinity of the channel for these ions is in the reverse order.
