Two Enzyme-Linked Immunosorbent Assay (ELISA) Systems for N¹, N⁸-Diacetylspermidine and N¹, N¹²-Diacetylspermine Using Monoclonal Antibodies

抄録

We obtained monoclonal antibodies against N¹, N¹²-diacetylspermine (DiAcSpm) and N¹, N⁸-diacetylspermidine (DiAcSpd), and developed two systems of competitive ELISA that utilize the antibodies and a common enzyme-labeled antigen to measure these diacetylpolyamines. Cross-reactions with N¹-acetylspermidine in the assay of DiAcSpm and with N⁸-acetylspermidine in the assay of DiAcSpd were as low as 0.26 and 0.6%, respectively, and were judged to be insignificant in clinical use for measuring urinary diacetylpolyamines. These assays were used to assess diurnal variations in diacetylpolyamine excretion in urine to show that the excretion of diacetylpolyamines after normalization for the concentration of creatinine is stable over a day with only minimal diurnal variation.