抄録
(1) L-ascorbic acid and L-cysteine hydrochloride are able to liberate iron from ferritin in vitro.
(2) The liberation of iron from ferritin is performed by some enzymic process in liver which is inhibited markedly by sodium fluoride, monoiodoacetic acid, pteroylglutamic acid, but slightly by potassium cyanide in the concentration of M/1000 and not by malonic acid.
(3) Hypoxanthine-xanthine oxidase system is able to liberate iron from ferritin under anaerobic circumstances, especially in the presence of methylene blue, but only slightly under aerobic condi-tions. Reduced diphospyridine nucleotide-diaphorase system exhibits the iron-liberating action upon ferritin. which is inhibited by ptero-ylglutamic acid.
(4) These evidences seem to demonstrate that diaphorase participates chiefly in the liberation of the ferritin iron under the physiological circumstances.
(5) It was proved that acetylcholine itself or even in the presence of liver brei has no apparent effect on ferritin.
Thanks are due to Prof. K. Kodama for his advice and Dr. H. Yoshikawa for his helpful suggestions.
The author gratefully acknowledge the gift of pteroylglutamic acid from Dr. H. Ninomiya of Takeda Pharm. Inc. Osaka.
