抄録
1. Crystalline beef liver catalase showed the peroxidatic activity with benzidine method, when the ratio of [hematin iron]/[hydrogen peroxide] was more than about 10-3.
2. From manometric experiments, very little peroxidatic oxidation of ethanol, and pyrogallol by catalase was demonstrated.
3. In the experiments of the peroxidatic oxidation of ethanol or of nitrite by catalase, the rate of the added hydrogen peroxide was controlled by a glass-needle with a capillary. Various concentrations of hydrogen peroxide were added continuously through the capillary at different rates. It is observed that “the slower is the addition of hydrogen peroxide, and also the lower is the concentration of added hydrogen perrxide, the more is the amount of ethanol or nitrite oxidiz-ed.”
4. The attempts to demonstrate the peroxidatic activity by attack-ing catalase molecule with heart, enzymes, urea, or cyanide, were un-successful.
5. The conditions of the peroxidatic reaction of catalase with hydrogen peroxide obtained in this paper, were discussed in relation to the reaction mechanisms of catalase.
Tands are due to Prof. K. Kodama for his grateful advice and to Dr. S. Tanaka for his kind suggestions.
