STUDIES ON THE INTERACTION BETWEEN PYRIDINE-HEMIN AND HYDROGEN PEROXIDE OR OXYGEN

I. SPECTROSCOPIC OBSERVATIONS ON THE PROCESS OF VERDOHEMOCHROME FORMA-TION WITH SPECIAL REFERENCE TO ITS QUANTITATIVE TREATMENT

抄録

The reaction process of verdohemochrome formation from pyridine-hemin was spectroscopically studied, which takes place through the aeration in the presence of ascorbic acid. The results are summarized as follows:
1. The reaction in this system proceeds in two stages: pyridinehemin→630-compound (an intermediate substance giving its maximal absorption at 630mμ)→verdohemochrome.
2. 630-compound is formed by the action of H₂O₂ under the presence of ascorbic acid.
3. The process of 630 -compound→verdohemochrome requires particularly the presence of molecular oxygen.
4. Method of the quantitative analysis of the reaction process was established.
5. The reaction proceeds completely quantitatively throughout the whole process.