巻号一覧
38 巻, 3 号
選択された号の論文の11件中1～11を表示しています
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I. SPECTROSCOPIC OBSERVATIONS ON THE PROCESS OF VERDOHEMOCHROME FORMA-TION WITH SPECIAL REFERENCE TO ITS QUANTITATIVE TREATMENTKOOZOO KAZIRO, GORO KIKUCHI1951 年 38 巻 3 号 p. 213-224
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリーThe reaction process of verdohemochrome formation from pyridine-hemin was spectroscopically studied, which takes place through the aeration in the presence of ascorbic acid. The results are summarized as follows:
1. The reaction in this system proceeds in two stages: pyridinehemin→630-compound (an intermediate substance giving its maximal absorption at 630mμ)→verdohemochrome.
2. 630-compound is formed by the action of H2O2 under the presence of ascorbic acid.
3. The process of 630 -compound→verdohemochrome requires particularly the presence of molecular oxygen.
4. Method of the quantitative analysis of the reaction process was established.
5. The reaction proceeds completely quantitatively throughout the whole process.抄録全体を表示PDF形式でダウンロード (687K) -
RYOITI SHUKUYA1951 年 38 巻 3 号 p. 225-236
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. As to the human blood cholinesterase, the relationship between enzymes and substrate was analyzed from a kinetic point of view, in order to explain the presentation of difference in erythrocyte and serum cholinesterase which have been expressed by the pS-activity curve.
2. Michaelis constants for erythrocyte-and-serum cholinesterase has been calculated as 3.7×10-4mole/lit. and 1.2×10-3mole/lit. respectively.
3. The inhibition by excess of substrate is subject to formation of ESS inactive complex produced by the combination of one molecule of enzyme with two molecules of substrate. The dissociation constant of ESS for erythrocyte cholinesterase was calculated as 1.2×10-2mole/l.
4. The inhibition by eserine is caused by the combination of one molecule of eserine with one molecule of enzyme, and the dissociation constants for erythrocyte and serum cholinesterase-eserine complexes were calculated as 0.88×10-8mole/lit. and 0.91×10-8mole/lit. respectively.
5. The inhibition of erythrocyte and serum cholinesterase by eserine is subject fundamentally to the same process.抄録全体を表示PDF形式でダウンロード (643K) -
SUZUOKI ZIRÔ, SUZUOKI TUNEKO1951 年 38 巻 3 号 p. 237-254
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリーUsing the suspension of Trichomonas foetas, one of the polyflagellated protozoa, the carbohydrate metabolism was studied manometrically under both aerobic and anaerobic conditions and, the biochemical changes during the course of the anaerobic culture were observed.
1. The preliminary tests were carried out in order to determine the standard conditions for the study of glucose oxidation, that is, with respect to the effects of pH, salt concentration, glucose concentration and the numbers of the parasite on the oxygen uptake of T. foetus.
2. Of the twenty seven substrates tested, only glucose, fructose, mannose, galactose, , sucrose and maltose increased the oxygen uptake of the parasite, while the others.did not evidently activate the endogene ous respiration. Especially, lactate, pyruvate and C4-dicarboxylic acids did not influence the respiration of the parasite, and malonate had no inhibitory effect on the oxygen uptake. These facts suggested that Krebs' tricarboxylic acid cycle did not take part in this aerobic carbohydrate metabolism.
3. The facts that the respiration of this protozoon was strongly inhibited by monoiodoacetate (0.00005M) and sodium fluoride (0.001M), but not by cyanide (0.01M) and azide (0.1M) and that acid formation was observed in the aerobic breakdown of glucose, gave the suggestion that the Embden-Meyerhof-Parnas' glycolytic process has relation to this glucose metabolism.
We demonstrated the presence of catalase and peroxidase in minced juice of the parasites and also ascertained spectroscopically the existence of cytochrome b in T. foetus, but could find neither the absorption bands of cytochrome a and c nor that of cytochrome oxidase.
4. In the anaerobic breakdown of glucose, the parasite evolved hydrogen gas as well as metabolic carbon dioxide, and produced a large quantity of acids, of which more than 70% was identified as succinic acid. Both lactic and pyruvic acids were scarcely produced in the same condition. In these facts, this anaerobic glucose metabolism differed considerably from the usual glycolysis.
5. During the course of the anaerobic cultivation of this protozoon, the titrable acids formation proceeded in parallel with glucose consumption, while the parasites were developing. Of the total amount of the titrable acids, that of succinic acid was 83%, but that of lactic and pyruvic acids, less than 10%. The gas produced in the cultivation was shown by micro gas analysis to contain a large amount of hydrogen and a small amount of methane.
We are deeply indebted to Prof. K. Okunuki for the spectroscopic observation and to Dr. C. Koyama for micro gas analyses and to Mr. H. Huzitani for the cultivation of the parasite. The authors' thanks are also due to Prof. F. Egami for his continued interest and advice.抄録全体を表示PDF形式でダウンロード (984K) -
SIGEHIRO MORITA, KOICHI SUZUKI, SHIRO TAKASHIMA1951 年 38 巻 3 号 p. 255-262
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. It was found that a non-sulfur purple bacterium, Rhodabacillus palustris evolves molecular hydrogen when illuminated white weak light This process was shown to be the cause of the peculiar relationship found _between the light intensity and the apparent rate of photosynthesis in purple bacteria.
2. The effect of various factors upon the process of photochemical H2-evolution were investigated. It is suppressed by hydroxylamine, by treatment of bacterial cells with ultraviolet rays, by heating at 45°., and also retarded to some extent, but not completely, by ammonium ion and, presumably, by molecular nitrogen. Acceleration of the process was brought about by provision of fumarate, succinate or pyruvate to bacterial suspension, while no such effect was observed with malate.
3. Using butyrate as a hydrogen-donor and eliminating the effect of H2-production by placing palladium asbestos in the manometer vessel, the rate of photosynthetic CO2-absorption was measured under various light intensities. The relationship thus found between the photosynthetic rate and the light intensity was essentially the same as that observed in green algae. A close analogy between the photosynthesis by purple bacteria and by green algae was also found in the temperature-dependency of the dark reaction, suggesting in both cases that the reaction involves at least two consecutive steps with widely different temperature coefficients. From the data obtained with the purple bacterium, the activation energies of these two reaction steps were computed. The values obtained (5-6k cal. and 26-27k cal.) were practically equal to these found earlier by Tamiya et al. for Chlorella ellipsoidea.
This work was carried out as part of the program directed by Professor H. Tamiya supported by The Scientific Research Expenditure of the Ministry of Education.抄録全体を表示PDF形式でダウンロード (572K) -
AKIJI FUJITA, MASATARO AOYAMA1951 年 38 巻 3 号 p. 263-269
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリーThe “activation” of GDH of Sobel is nothing other than the effect of SbCl3 which is contained in the reagents used. By the addition of an adequate amount of antimony trichloride and hydrochloric acid to GDH, an exact method of the vitamin A determination is established. This method has higher sensitivity than any other GDH methods hitherto reported, the merits of GDH methods being retained at the same time. The procedure and effects of various conditions were reported in detail.抄録全体を表示PDF形式でダウンロード (341K) -
AKIJI FUJITA, MASATARO AOYAMA1951 年 38 巻 3 号 p. 271-275
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (273K) -
II. ON THE HISTIDINE ACTIVATION OF PANCREAS LIPASETOSHIICHI YAMAMOTO1951 年 38 巻 3 号 p. 277-287
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. The acetone powder of pig pancreas was extracted with 70% glycerol water, and the supernatant fluid was used as the lipase solution in the experiments. L-Histidine solution stimulates the lipase action at pH 8.6 to about 56% for triacetin and 70% for tributyrin hydrolysis, but not for butyl-butyrate.
2. No difference is found between L- and DL-histidine in respect to its activation of the pancreas lipase in the range of from 0.005 to 0.04M final concentration of hisitdine, showing parallelism between the activation and the histidine concentration. There exists a relationship between the optimal concentrations of histidine and the maximal hydrolysis of triacetin and olive oil.
3. Regarding the activating effect of amino acids and their amine derivatives, effect of histamine (about 30%) is lower than histidine (70%), and that of tyramine is not ascertained, while tyrosine has yet some effect (about 30%).
4. The activity of the lipase solution (extract from pig pancreas powder giving a strong Pauli's diazo reaction) becomes after dialysis less than 10% of the original degree of the hydrolysis and it is reactivated by the addition of the concentrated dialysate, and also by the addition of L-Histidine solution. L-Histidine picrate was isolated from the con-centrated dialysate.
5. With the crude maceration of the fresh pig pancreas a weak imulating effect of histidine was found.
The author wishes to express his deepest gratitude to Dr. S. Utzino, Professor at the Faculty of Medicine, Kyoto University, for his kind constant guidance in this research. These investigations owed much to the aid-grants by the Ministry of Education for the Scientific Researches, for which author's thanks are here expressed.抄録全体を表示PDF形式でダウンロード (694K) -
MASAMI SUDA, KIZO HASHIMOTO, HIROSHI MATSUOKA, TAKESHI KAMAHORA1951 年 38 巻 3 号 p. 289-296
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. The further study of the pyrocatecase catalyzing breakdown of catechol has shown that one of the essential compentents of this enzyme is ferrous ion.
2. The end product of pyrocatecase reaction may be cis-cis muconic acid as reported earlier. By use of dried cells adapted to catechol, and cell extracts, cis-cis muconic acid was shown to be converted to β-ketoadipicacid.抄録全体を表示PDF形式でダウンロード (421K) -
III. RELATION BETWEEN HOMOGENTISICASE, FERROUS ION AND L-ASCORBIC ACID IN EXPERIMENTAL ALCAPTONURIA OF GUINEA PIGMASAMI SUDA, YOSHIRO TAKEDA, KIYOSHI SUJISHI, TAKEHIKO TANAKA1951 年 38 巻 3 号 p. 297-302
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリー1. The activity of homogentisicase was found to be remarkably lower in the liver of scorbutic guinea pig than in the liver of control animals. This low activity could be recovered by the addition of ferrous ion or L-ascorbic acid, the addition of the latter having proved to be far less effective.
2. In the guinea pig treated with αα'-dipyridyl, homogentisic acid is excreted in urine following the administration of L-tyrosine.
3. The homogentisicase activity in liver of guinea pigs treated with αα'-dipyridyl, is remarkably lower than the control. This low activity is recovered only when ferrous ion in added to the enzyme solution. L-ascorbic acid has no effect.
4. A function of L-ascorbic acid is animal body is discussed.抄録全体を表示PDF形式でダウンロード (331K) -
TOMIO OGATA, TAKEHIKO SHIMANOUCHI, SEIZABURO YAMAOKA1951 年 38 巻 3 号 p. 303-308
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリーThe quantitative observations were made on the histamine contraction of the guinea pig ileum strip at lower temperatures with various concentrations.
The results obtained by the Magnus technic at various histamine concentrations can be explained by the Langmuir's adsorption equation with the assumption that histamine is adsorbed on to the active sites of the smooth muscle.
Based on the data obtained at lower temcratures suggestion was made on the possibility of calculation of the adsorption energy of histamine to the muscle strip that was gauged to be somewhat around 10K cal.
The authors wish to express their deep appreciation of the kindnesses of Dr. Dan H. Campbell and his associates for the criticism of this work. Thanks are also due to Dr. San-ichiro Mizushima, Professor of Chemistry, and to Dr. Toshihiko Tokisane of the Department of Physiology, the University of Tokyo, for their valuable discussions.抄録全体を表示PDF形式でダウンロード (312K) -
1951 年 38 巻 3 号 p. e1
発行日: 1951年
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (11K)
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