抄録
1. A colorimetric quantitative determination of liver glucose de-hydrogenase activity was attempted by use of 2, 3, 5-triphenyltetrazolium bromide as hydrogen acceptor.
2. Reduction of TTB was carried out anaerobically in Thun-bergtubes.
3. At pH 7.4, the Michaelis constant (Km) of the enzyme was 0.075 or 0.085M according as Michaelis-Menten's or Line-weaver-Burk's method was employed.
4. DPN was necessary as hydrogen carrier between glucose de-hydrogenase and terminal hydrogen acceptors.
5. It may appear that flavoprotein (Straub's) may act as hydro-gen carrier between reduced DPN and TTB which functions as a hydro-gen acceptor.
The author is indebted to Dr. Y. Oshima and Dr. M. Shirakawa, of the Laboratory of Biochemistry, Faculty of Agriculture, and Dr. T. Misao, of the First internal Clinic, Faculty of Medicine, Kyushu University, who suggested the problem and gave many valuable advices.
