PARA-NITROPHENYL SULFATE HYDROLYSIS AND TRANSSULFATION TO CHARONINSULFURIC ACID BY CELL-FREE EXTRACTS OF “CHARONIA LAMPAS”
1959 年 46 巻 1 号 p. 1-10
詳細
1959 年 46 巻 1 号 p. 1-10
1. A method of synthesis of p-nitrophenyl S35-sulfate was described.
2. Cell-free extract of the mucous gland of “Charonia lampas” has been found to incorporate radioactive sulfate into charoninsulfuric acid when incubated with p-nitrophenyl S35-sulfate.
3. Two arylsulfatases with different pH optimum (5.0 and 6.0) were found in the extract of mucous gland and separated by zone electrophoresis. These arylsulfatases were not able to transfer sulfate from phenol to carbohydrate.
4. Arylsulfatase of the liver of Charonia lamfias was purified. The purified arylsulfatase preparation has been found to catalyze the incorpora-tion of S35-sulfate to polysaccharide in the presence of a non-dialyzed preparation of charoninsulfuric acid and p-nitrophenyl S35-sulfate. This activity has not been observed with dialyzed charoninsulfuric acid. Some unknown factor may participate in the reaction.
A part of the expense of this study was defrayed by a grant from Seikagaku-Kenkyusho Ltd., to which our thanks are due. Some of the experiments were carried out in the Marine Biological Station of Nagoya University.
