The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Studies on the Firmly Bound Lipids of Human Tubercle Bacillus
II. Isolation of Arabinose Mycolate and Identification of Its Chemical Structure
ICHIRO AZUMAYUICHI YAMAMURA
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1963 年 53 巻 4 号 p. 275-281

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1. A new glycolipid, arabinose mycolate, was isolated from the B fraction of bound lipids of human tubercle bacillus Aoyama B strain after treatment of 0.1 N HCl at 37°C for 72 hours.
2. By acid and alkaline hydrolysis of this glycolipid, mycolic acid and D-arabinose were obtained. Mycoloyl alcohol was obtained by reductive cleavage with LiAlH4.
3. Methylation showed that one molecule of mycolic acid and one molecule of D-arabi-nose are attached through an ester linkage to 5 hydroxy group of D-arabinose.
The authors express their deep gratitude to Prof. T. Momose and Prof. H. Tsukamoto, School of Pharmacy, Kyushu University, for their helpful sug-gestion and Prof. M. Tomoda, Kyoritsu Pharmaceu-tical College for kind supplement of the samples of methylated sugar. The authors are indepted to Mrs. S. Matsuba and Mr. M. Shidoh for elementary analysis, and Mr. K. Hikita for spectral measurement. This work was supported by a grant for scientific researchs from the Ministry of Education.
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© The Japanese Biochemical Society
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