Enzymatic Studies on the Formation of 5-Ketogluconic Acid by Acetobacter suboxydans
I. Glucose Dehydrogenase
1963 年 53 巻 5 号 p. 348-353
詳細
1963 年 53 巻 5 号 p. 348-353
The primary step for the formation of 5KG1cA from glucose was studied with the soluble fraction of Acetobacter suboxydans. The enzyme, glucose dehydrogenase, was separated from 5KG1cA reductase and was shown to be exclusively NADP-linked. The optimum pH was about 8.5. Of the several substrate ex-amined, only glucose and mannose were dehydrogenated by this enzyme and were proved to give corresponding aldonic acids stoichiometrically. Some properties of the enzyme were compared with that of particu-late enzyme.
The author wishes to express his grateful thanks to Prof. S. Tanaka for his interest and encourage-ment.
