Preparation and Some Properties of α-Actinin-free Actin
1965 年 58 巻 1 号 p. 20-26
詳細
1965 年 58 巻 1 号 p. 20-26
1. The method for preparation of α-actinin-free actin from exhaustively washed muscle residue was described.
2. α-Actinin-free F-actin thus prepared has a long particle length corresponding to Straub-type actin, but it does not show the tendency of network formation.
3. Physico-chemical properties of α-actinin-free actin subjected to trypsin digestion
or to repeated polymerization-depolymerization combined with sedimentation at high speed centrifugation became similar to those of Straub-type actin.
4. It was concluded that α-actinin-free actin contained β-actinin and the latter was eliminated by the above procedures.
5. Superprecipitation of actomyosin con-sisting of α-actinin-free actin was not influ-enced by the presence of β-actinin.
