巻号一覧
58 巻, 1 号
選択された号の論文の17件中1～17を表示しています
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II. Inhibition of the Enzyme Activity by NAD and Stimulation of That by p-ChloromercuribenzoateEIICHI MISAKA, KAZUO NAKANISHI1965 年 58 巻 1 号 p. 1-6
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリーMenadione reductase of bakers' yeast is inhibited by NAD, non-competitively, and promotion of the enzyme activity occurs by PCMB-treatment.
This enzyme has two sulfhydryl groups which are determined by PCMB-method. One of them is supposed to be highly active and reacts with PCMB at low concentration. The other one is thought to be sluggish in reactivity and reacts only with PCMB at high concentra-tion. When the first active sulfhydryl group is filled with PCMB, the slight inhibition of the enzyme activity occurs. But when the second sluggish one is also filled with PCMB, promotion of the enzyme activity takes place. It is suggested that PCMB dependent inhibi-tion and promotion are caused by some con-formational changes of the enzyme protein pro-duced by mercaptide linkages between PCMB and the sulfhydryl groups of the enzyme.
The authors wish to thank Dr. M. Matsui, the Director of the Laboratories, for his encouragement of the work during the course of this investigation and Dr. M. Nakamura, the Superintendent of Sankyo Tanashi Plant, for the supply of a large amount of the enzyme source.抄録全体を表示PDF形式でダウンロード (412K) -
I. Preparation and Action on Actomyosin-ATP InteractionSETSURO EBASHI, FUMIKO EBASHI1965 年 58 巻 1 号 p. 7-12
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリー1. A new protein, named α-actinin, was extracted from rabbit skeletal muscle and its effect on the shrinking process of actomyosin was studied by turbidometric method.
2. α-Actinin has a remarkable accelera-ting effect on the superprecipitation process of actomyosin. The presence of α-actinin allowed superprecipitation to take place at higher ionic strength and increased the degree of shrinking of actomyosin.
3. Usual actin preparation was shown to contain more or less α-actinin. Interaction between myosin and α-actinin-free actin was very weak under the usual conceneration of ATP, i.e., superprecipitation was hardly obser-vable under this condition. Addition of α-actinin greatly strengthened the interaction between actin and myosin and gave rise to a remarkable superprecipitation.
4. The amino acid composition of α-actinin resembles that of actin. Some other properties of α-actinin are also somewhat similar to those of denatured actin. However, the denatured actin did not show any promo-ting effect on superprecipition.
5. The role of α-actinin in the mechanism of muscular contraction is discussed.
We would like to express our sincere thanks to Dr. K. Maruyama for his advice and discussions. Our thanks are also due to Miss A. Kodama for her technical assistance.抄録全体を表示PDF形式でダウンロード (1339K) -
II. Action on ActinKOSCAK MARUYAMA, SETSURO EBASHI1965 年 58 巻 1 号 p. 13-19
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリー1. The gelation of F-actin was evoked by adding an appropriate amount of α-actinin. Larger amounts of a-actinin co-precipitated F-actin. It is concluded that ‘gel-actin’ is a complex of F-actin and α-actinin.
2. Polymerization rate of G-actin was accelerated by a-actinin when observed with viscometry or with flow birefringence.
3. Effects of α-actinin alone or with β-actinin on the birefringent properties of F-actin are described.
4. Correlation between the effects of α-actinin on the physical properties of actin and its effect on superprecipitation is discussed.抄録全体を表示PDF形式でダウンロード (1016K) -
SETSURO EBASHI, KOSCAK MARUYAMA1965 年 58 巻 1 号 p. 20-26
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリー1. The method for preparation of α-actinin-free actin from exhaustively washed muscle residue was described.
2. α-Actinin-free F-actin thus prepared has a long particle length corresponding to Straub-type actin, but it does not show the tendency of network formation.
3. Physico-chemical properties of α-actinin-free actin subjected to trypsin digestion
or to repeated polymerization-depolymerization combined with sedimentation at high speed centrifugation became similar to those of Straub-type actin.
4. It was concluded that α-actinin-free actin contained β-actinin and the latter was eliminated by the above procedures.
5. Superprecipitation of actomyosin con-sisting of α-actinin-free actin was not influ-enced by the presence of β-actinin.抄録全体を表示PDF形式でダウンロード (1156K) -
LXXII. Fecal Bile Acids in the DogSHIGEO HIROFUJI1965 年 58 巻 1 号 p. 27-33
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (358K) -
KAZUO HAYANO, FUMIO ITO, YUICHI YAMAMURA1965 年 58 巻 1 号 p. 34-38
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリーLiver lipid content of rats fed a 0.3% 2-ethylthioisonicotinamide diet for one week in-creased to about twice that of rats on a control diet. Accumulated lipids were chiefly triglycerides.
Intraperitoneal injection of phosphoryl-choline, cytidine and 5-aminoimidazole-4-car-boxamide showed complete inhibition of 2-ethylthioisonicotinamide fatty liver, while cytidine diphosphate choline, aspartylcholine and uridine also showed the considerable inhibition but not to the level of lipid content in the control group. The preventive effect to the 2-ethylthioisonicotinamide fatty liver of choline chloride, methylglycine, earni-thine, ethanolamine and orotic acid was not observed.
The mechanism of induction of 2-ethyl-thioisonicotinamide fatty liver was discussed.抄録全体を表示PDF形式でダウンロード (304K) -
MIHOKO YOSHIDA, NORIO SHIMAZONO1965 年 58 巻 1 号 p. 39-46
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリー1. Column- and paper-chromatographic methods for the separation of hemin a and protohemin from heart muscle extracts were described. Silicic acid impregnated paper and alumina impregnated paper were used for paper chromatography. Alumina was used for column chromatography.
2. Hemin a and protohemin could be separated effectively on silicic acid impregna-ted paper with ethanol-water-formic acid or acetone-water-formic acid as the solvent system. Effective separation was also observed on alumina impregnated paper with ethanol-water-formic acid as the solvent system. In all cases, the Rf value of hemin a was higher than that of protohemin.
3. There was essentially no tailing with these methods. The degree of separation was dependent on the concentrations of both water and formic acid.
4. A separation was observed on alumina impregnated paper with chloroform-formic acid or chloroform-acetic acid as the solvent system. However, in these cases, the Rf values of hemin a were smaller than those of protohemin.
5. Hemin a was separated on a alumina column, using a acetone-water-formic acid mixture as eluant. Hemin a was eluted prior to protohemin by this method. However, as the hemin a fraction and the protohemin frac-tion overlapped considerably, rechromato-graphy was necessary to obtain pure hemin a.
6. The spectrophotometrical properties of hemin a obtained by this method were identical with those of hemin a from purified cyto-chrome c oxidase and also agreed with the descriptions of many previous investigators. But no substance corresponding to the hemin A reported by Caughey and York was found during this work.
The authors express their deep gratitude to Dr. S. Horie of this Department for his valuable advice.抄録全体を表示PDF形式でダウンロード (569K) -
II. Isocitrate Dehydrogenase and Transhydrogenase in Tumor Bearing Rat Liver and Ascites Tumor CellsTORU OTANI, TANEAKI HIGASHI, ETSUKO MARUYAMA, YUKIYA SAKAMOTO1965 年 58 巻 1 号 p. 47-52
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリーActivities and properties of two NADP-linked isocitrate dehydrogenases which differ in its location, and transhydrogenase are ex-amined in tumor bearing rat liver and ascites tumor cells. Neither isocitrate dehydrogenase nor transhydrogenase of liver showed any significant change in specific activity during tumor growth, but the activities of these enzymes per unit DNA decreased daily.
There was no significant difference bet-ween the specific activities of AH-130 tumor cells' mitochondrial enzyme and the liver mitochondrial enzyme on the basis of mito-chondrial protein, but the soluble enzyme of tumor cells showed only one fifth to one tenth of the activity of that of the liver.
Isocitrate dehydrogenase in the AH-130 tumor cells showed and lower sensitivity to PCMB than that in the liver. Soluble iso-citrate dehydrogenase showed about two-fold sensitivity to PCMB, compared with mito-chondria) isocitrate dehydrogenase in both the AH-130 tumor cells and the liver.
Adenine nucleotides have no significant effect on the enzyme activity.抄録全体を表示PDF形式でダウンロード (441K) -
TAKAO MINAMIKAWA, IKUZO URITANI1965 年 58 巻 1 号 p. 53-59
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリーMode of inhibition of some conceivable intermediates of chlorogenic acid synthesis and other related compounds on the activity of phenylalanine ammonia-lyase A and B of sweet potato roots were investigated.
Among the phenylproponoids examined, trans-cinnamic, p-eoumaric and caffeic acids showed competitive inhibition on phenylalanine ammonia-lyase A, and trans-cinnamic acid in-hibited competitively the activity of phenyl-alanine ammonia-lyase B. trans-Cinnamic acid which is the direct product of the deamina-tion reaction, was found to be the most potent inhibitor of the two enzymes. DL-p-Fluoro-phenylalanine and L-tyrosine were also com-petitive inhibitors in both cases. The inhibitor constants for these competitive inhibitors were calculated. Choorogenic and isochlorogenic acids, the major polyphenols accumulated in sweet potato roots, showed no inhibitory effect on the enzyme activity. Quinic acid, the non-aromatic moiety of chlorogenic acid, was not effective on the inhibitory action of the phenolic inhibitors, and quinic acid itself did not show inhibition on the enzyme activity.抄録全体を表示PDF形式でダウンロード (373K) -
HIROSHI HASHIZUME, KAZUTOMO IMAHORI1965 年 58 巻 1 号 p. 60-67
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリー1. The activation energy of tryptic activa-tion of a-chymotrypsinogen was obtained by the enzyme assay and ultraviolet absorption spectral change. The values obtaind by the two methods were fairly in good agreement.
2. The spectrophotometric titration on both α-chymotrypsinogen and π-chymotrypsin revealed that two out of the total four tyrosyl titratable.
3. Two exposed tyrosyl residues were easily iodinated both in π-chymotrypsin and a-chymotrypsinogen. Iodinated samples were in full activity or activatability.
4. Two exposed tyrosyl residues were easily modified with FDNB. Since the spectral change observed during the tryptic activation of DNP-chymotrypsinogen was the same as that observed in tryptic activation of a-chymotrypsinogen, this spectral change was attributed to the environmental change of the hurried tyrosyl residue.抄録全体を表示PDF形式でダウンロード (545K) -
III. Effect of Insulin on Lipid Synthesis in Dispersed Rat Liver CellsKOZABURO ADACHI, HIDEO INOUE, HIROAKI TANIOKA, YOSHIRO TAKEDA1965 年 58 巻 1 号 p. 68-72
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (328K) -
TAKAHISA OHTA, YASUYUKI OGURA1965 年 58 巻 1 号 p. 73-89
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリーThe action of yeast alcohol dehydrogenase was inhibited reversibly by the presence of urea less than 2M. From kinetic data, it was assumed that urea does not compete with either substrate or coenzyme. The results of sedimentation analysis suggested that the reversible dissociation of the enzyme molecule into four subunits was caused by the presence of urea less than 2M. The enzymatic reac-tion, however, was inhibited irreversibly by concentrations of urea more than 2M. From measurements of ultraviolet difference spectra, fluorescence spectra and viscosity, the unfold-ing of the enzyme molecule was suggested to occur under these conditions. A good agree-ment was found between the change in protein structure and the irreversible loss of activity.抄録全体を表示PDF形式でダウンロード (2135K) -
KAZUO SATAKE, ATSUKO MATSUO, TERUO TAKE1965 年 58 巻 1 号 p. 90-97
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリーAn automatic analyzer of amino acids and lower peptides with 1-fluoro-2, 4-dinitro-benzene was constructed. The instrument could be used both for quantitative monitor-ing their chromatographic separation and for discontinuous analysis of previously fraction-collected samples with a high velocity. The maintenance was rather simple and only one gram of the reagent permitted continuous analysis over 30 hours. Samples after analysis could be recovered as their dinitrophenyl derivatives, easily.抄録全体を表示PDF形式でダウンロード (526K) -
KAZUO MOTONAGA, KAZUO NAKANISHI1965 年 58 巻 1 号 p. 98-100
発行日: 1965/07/25
公開日: 2008/11/18
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CHING TSANG HOU, KEIJI HARASHIMA, SABURO FUNAHASHI1965 年 58 巻 1 号 p. 101-103
発行日: 1965/07/25
公開日: 2008/11/18
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RYUZO SADAHIRO, SHIGERU TAKANASHI, MINORU KAWADA1965 年 58 巻 1 号 p. 104-106
発行日: 1965/07/25
公開日: 2008/11/18
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SETSURO EBASHI, AYAKO KODAMA1965 年 58 巻 1 号 p. 107-108
発行日: 1965/07/25
公開日: 2008/11/18
ジャーナル フリーPDF形式でダウンロード (103K)
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