抄録
1. Glycopeptides prepared from ovalbumin and orosomucoid according to the methods as published previously were hydrolyzed patially with acid and the hydrolysates were fractiona-ted by means of column chromatography on Dowex-50 in pyridine-acetic acid system. The front peaks were further fractionated on Dowex-50 in H+ form and ninhydrin positive materials could be freed from neutral sugars.
2. From ovalbumin, two compounds could be isolated of which one was the primary product of the hydrolysis and the other its -derivative. The primary product was found to be indistinguishable from a synthetic aspa-raginylglucosamine (1-L-β-aspartamido-2-aceta-mido-1, 2-dideoxy-β-D-glucose) in the behav-iours in chromatography, electrophoresis, nin-hydrin reaction on paper and infrared spectro-scopy. Thus, it was concluded that β-carboxyl of aspartic acid is linked to 1-amino group of 2-deoxy-2-acetamidoglucosylamine.
3. From orosomucoid, the same aspara-ginylglucosamine could be isolated. However, three other peaks were also obtained in the final chromatography of which only one was identified as threonine. Possibility of the presence of other linkage than the asparagine-glucosamine in this glycoprotein was discussed.
4. A new route for the synthesis of the .asparaginylglucosamine was presented.
