Binding of D-Glucuronic Acid with Ovalbumin

II. Immunological Properties of the Compound

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Using OA and OA-G as antigens, antisera were obtained from rabbits. Anti-OA and anti-OA-G serum were submitted to various immunological tests against OA and OA-G, such as tube precipitin test, precipitation test by double diffusion in agar gel, immunoelec-trophoresis, and specific absorption test. By tube precipitin test, it was observed that anti-OA and anti-OA-G serum were cross-reacted against OA and OA-G respectively. Precipita-tion by double diffusion in agar gel did not revealed any antigenic difference between OA and OA-G. On immunoelectrophoresis, when OA and OA-G were separated electrophoreti-cally in an agar layer and antisera were intro-duced into the horizontal trough, the specific precipitin are was each single. Furthermore, anti-OA-G serum, after it was absorbed with OA, gave no specific precipitate with OA-G.
Consequently, no notable difference was observed antigenic properties between OA and OA-G.
The authors express our gratitude to Dr. T. Sawada, Japan BCG Laboratory and Dr. K. Yusawa, Research Institute of Tuberculosis, Japan Antituber-culosis Association for biological assistance during this study and thanks are also due to Miss H. Ushiyama and Mrs. N. Matsuzawa, for supplying the antisera.