Biochemistry of Shellfish Lipid
III. Purification and Elemental Analysis of Ceramide Aminoethylphosphonate from Corbicula Complex Lipid Mixtures
1966 年 59 巻 6 号 p. 570-573
詳細
1966 年 59 巻 6 号 p. 570-573
Purification of ceramide aminoethyl-phosphonate from the corbicula, Corbicula
sandai was accomplished by the use of silicic acid column chromatography and by the application of mild alkaline hydrolysis of Dawson. Thus it was found that the pure lipid can be more effectively prepared by the alkaline hydrolysis according to the D a w s o n method than by the silicic acid column chromatography.
In addition, some properties and ana-lytical values of the lipid purified by the alkaline hydrolysis method are presented, and they essentially agreed with those estimated for ceramide aminoethylphos-phonate.
The authors express their cordial thanks to Prof. T. Yamakawa of the University of Tokyo for his kind advice and encouragement.
