Degradation of RNA Polymerase in Escherichia coli
1967 年 61 巻 3 号 p. 359-366
詳細
1967 年 61 巻 3 号 p. 359-366
Experimental evidence was presented indicating that the loss of DNA dependent RNA polymerase [EC 2. 7. 7. 6] activity in E. cola which is infected with bacteriophage T2 is due to the degradation of enzyme molecule detached from the host DNA. An enzyme preparation extracted from a deoxyribonucleoprotein-protamine complex was used as a model undergoing degradation with a loss of enzyme activity. The degradation profile of the enzyme in the preparation was studied with special reference to the changes of its molecular size and activity. The native enzyme molecule showed a sedimentation constant of 16S. On storage at 2°C, the activity of the enzyme decreased gradually and the enzyme molecule degraded to give rise to smaller subunits with release of some component. Addition of this component to the inactivated enzyme resulted in the complete recovery of the enzyme activity.
