A Glycoprotein from Bovine Nasal Cartilage Preparation Methods and Properties
1972 年 71 巻 4 号 p. 635-643
詳細
1972 年 71 巻 4 号 p. 635-643
A glycoprotein has been isolated from bovine nasal cartilage by various methods and its chemical and physicochemical properties were examined.
1. Analytical disc electrophoresis on acrylamide gel shows that, besides the major component, a number of minor components are also present.
2. The relative proportions of these minor fractions increase with time. The presence of large amounts of half-cystine residues in the peptide portion suggests that the minor fractions could be formed from the main component by rearrangements in-volving disulphide bonds. This tentative suggestion still requires proof.
3. The glycoprotein contains approximately 92% of protein, 3% hexosamine, 1% uronic acid and 2% neutral sugars. The uronic acid component appears to be part of a glycosaminoglycuronoglycan structure which is an integral part of the glyco-protein molecule.
4. The glycoprotein has a probable molecular weight near 45, 000.
