Studies on the Substrate Specificity of Taka-amylase A

XI. Effect of Release of p-Phenylazobenzoyl Residue from p-Phenylazobenzoyl Taka-amylase A on the Enzymatic Action

抄録

p-Phenylazobenzoyl Taka-amylase A (PhAB-TAA) has much less amylase activity and an elevated phenyl maltosidase activity as compared with the intact Takaamylase A (TAA).
When the p-phenylazobenzoylated enzyme (PhAB-TAA) was stored at 0-4°C at pH 6.0 in the dark, a part of p-phenylazobenzoyl residues that had been introduced into Taka-amylase A (TAA)[EC 3. 2. 1. 1] was slowly liberated from the enzyme.
On liberation of the phenylazobenzoyl residue the action pattern of PhAB-TAA gradually changed to that of the intact TAA.