Studies on Lipolysis in Lipid Micelles Isolated from Adipose Tissue

抄録

Adrenaline-sensitive lipid micelles were prepared from rat epididymal adipose tissue by hypotonic treatment. The lipolytic activity (free fatty acid release) of these micelles was stimulated by DBcAMP, theophylline and caffeine, but not by cyclic 3', 5'-adenosine monophosphate (cyclic AMP), adenosine or adenosine monophosphate.
Lipolysis in lipid micelles was also increased by phospholipase C [EC 3. 1. 4. 3]
but not by phospholipase D [EC 3. 1. 4. 4].
When adrenaline and phospholipase C were incubated together with the lipid micelles, their effects were not additive.
The lipolytic activity of lipid micelles prepared from isolated fat cells which had been preincubated with 10μg per ml of adrenaline was higher than that of micelles preincubated without adrenaline and was not stimulated by further addition of adrenaline to the assay system.