A Simple Method for the Isolation of Actin from Myxomycete Plasmodia
1977 年 82 巻 1 号 p. 201-205
詳細
1977 年 82 巻 1 号 p. 201-205
A new, simple method for the isolation of actin from myxomycete plasmodia has been developed. Plasmodium myosin B was incubated at 55°C for 15min in the presence of ATP or was treated with 90% acetone. By this treatment myosin was denatured completely. Actin was then extracted with a dilute ATP and cysteine solution from the heat- or acetone-treated myosin B. The method is simple and almost pure actin was obtained in high yield.
The purified G-actin polymerized to F-actin on addition of 0.1M KCl or 2mM MgCl2. The viscosity of the purified F-actin was 8-10dl/g. The F-actin activated muscle myosin ATPase, and actomyosin synthesized from the F-actin and muscle myosin showed superprecipitation on addition of ATP.
