The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Calcium-Sensitivity of the Microtubule Reassembly System
Difference between Crude Brain Extract and Purified Microtubular Proteins
Eisuke NISHIDAHikoichi SAKAI
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1977 年 82 巻 1 号 p. 303-306

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Microtubule reassembly in crude extracts of porcine brain was inhibited by 10-5M Ca2+, whereas 10-3M Ca2+ was required for inhibition of the reassembly from purified microtubular proteins. This accounts for the apparent discrepancies between the results reported by other investigators. Furthermore, the Ca-sensitivity of the purified microtubular proteins was nearly completely recovered on addition of a fraction obtained from crude brain extract.
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© The Japanese Biochemical Society
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