The Journal of Biochemistry
Online ISSN : 1756-2651
Print ISSN : 0021-924X
Gangliosides and Neutral Glycolipids in Guinea Pig Adrenal Glands
Michiko SEKINEToshio ARIGATadashi MIYATAKERyoichi KASEAkemi SUZUKITamio YAMAKAWA
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1984 年 96 巻 1 号 p. 237-244

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Glycolipids were isolated from the adrenal glands of seven strains of guinea pig by DEAE-Sephadex and Iatrobeads column chromatographies. The average lipidbound sialic acid content of the adrenal glands was estimated to be 96.0±30.4 nmol/g fresh tissue. The ganglioside fraction contained two major gangliosides which accounted for 82% of the total lipid-bound sialic acid. They were identified as N-acetylneuraminylgalactosylceramide (GM 4) and N-acetylneuraminyllactosylceramide (GM 3). The neutral glycolipid fraction contained one major and three minor glycolipids. The major glycolipid was identified as galactosylceramide, which accounted for 81% of the total glycolipid. The other three glycolipids were identified as Forssman glycolipid (7%), lactosylceramide (5%), and gangliotriaosylceramide (7%). In all strains examined, the glycolipid patterns were similar.
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© The Japanese Biochemical Society
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