Comparison of &-D-Galactosidase from Escherichia coli and Horseradish Peroxidase as Labels of Anti-Human Ferritin Fab'by Sandwich Enzyme Immunoassay Technique

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β-D-Galactosidase from Escherichia coli and horseradish peroxidase were compared as labels of anti-human ferritin Fab' by sandwich enzyme immunoassay technique using fluorogenic substrates for enzyme assay. The anti-human ferritin Fab'-peroxidase conjugates gave lower nonspecific bindings and higher specific bindings than the corresponding Fab'-β-D-galactosidase conjugates. As a result, the former provided more sensitive dose response curves for human ferritin than the latter. However, the peroxidase conjugates were required in a larger quantity, since peroxi-dase assay was much less sensitive than β-D-galactosidase assay.