Granule formation of elongator-specific methionine transfer RNA caused by X-ray irradiation in a non-small lung carcinoma cell line (NCI-H1299)

抄録

We herein report the subcellular distribution of methionine transfer RNA (tRNA^Met), which has two forms of initiator tRNA^Met (Meti) and elongator tRNA^Met (Mete). Meti induces translation initiation, and Mete is responsible for the elongation of the peptide chain. A recent report showed that the formation of tRNA^Met granules occurs following heat shock stress. We analyzed the subcellular distribution change in tRNA^Met using ionizing irradiation. The images acquired using confocal microscopy showed the presence of Mete granules—but not Meti granules—in the cytoplasm. Furthermore, while the Mete granules co-localized with T-cell intracellular antigen-1, they did not co-localize to typical stress granules nor processing body specific markers.