Abstract
Local irrigation using ozone nano-bubble water (NBW3) with high anti-bacterial/cleaning effect and the mouthwash
method are applied clinically for the prevention of postoperative infection after implant surgery.
The present study investigated the optimal mouth washing time for killing Streptococcus mutans, initial
colonizers of biofilm formation when mouth washing using NBW3.
In addition, a commercially available mouthwash (chlorhexidine gluconate solution) was used to comparatively
review both antibacterial effects. The results showed that the decrease rate of viable bacteria using NBW3 had
reduced to less than about 10% and 1%, respectively, for 5 and 10 seconds, and to less than the detection limits
in the present study for 30 and 60 seconds. Also, that of viable bacteria using a chlorhexidine gluconate solution
had reduced to about 92, 81, 77, and 58%, respectively, for 5, 10, 30, and 60 seconds. The results showed higher
antibacterial activity of NBW3 in each duration of activity compared with ConCool F® (chlorhexidine gluconate
solution) under the same conditions of the present study.
The results of the present study suggest that mouth washing using NBW3 might kill intraoral bacteria more
efficiently compared with using a mouthwash solution.
