Preparation of Si-containing calcium phosphate thin film and evaluation of cell adhesion

Abstract

Ti implants have been used primarily for current dental implants．However the peri-implant inflammation is a serious ploblem．In order to improve the adhesion between the abutment and the epithelium，Si-containing calcium phosphate thin film containing Si-Ca-P was coated on Ti using sputtering method from a mixture of hydroxyapatite and SiO2 as a target．Then，cell adhesion on the thin film was evaluated．Mouse embryo derived fibroblasts (NIH-3T3) were seeded on Si-containing calcium phosphate thin films．After 48 hours of cell seeding, fluorescence image of cells and the number of attached cells were observed using a microscope． As a result，the number of attached cells on the Si- containing calcium phosphate thin films increased with increasing Si content，and reached a maximum of 46 (cells/frame) on the film with 30% of SiO2 /SiO2 +HA target．Then the number of attached cells decreased to 24 (cells/frame) with the film with 100% of SiO2 target．