抄録
The active site of the [NiFe] hydrogenase from Desulfovibrio vulgaris Miyazaki F in the oxidized form has been reported as a hetero binuclear Ni-Fe complex with four non-protein ligands (SO, CO, CN, S). In contrast, the non-protein ligands in the hydrogenase from Desulfovibrio gigas were reported as CO, CN, CN and O. The S bridging ligand atom was confirmed by two experiments that the hydrogenase liberates H2S upon reduction with H2 and that the crystal structure of the H2-reduced form lost the bridging ligand between the Ni and Fe atoms. The possible mechanism of the hydrogen activation is discussed.
