生物物理
Online ISSN : 1347-4219
Print ISSN : 0582-4052
ISSN-L : 0582-4052
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通巻350号
選択された号の論文の15件中1~15を表示しています
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  • 須藤 雄気, 小島 慧一
    2020 年 60 巻 4 号 p. 209-214
    発行日: 2020年
    公開日: 2020/07/29
    ジャーナル フリー

    One of the major topics in biophysics and physicobiology is to understand and utilize biological functions using various techniques. Rhodopsin is a photoreactive membrane-embedded protein that uses a retinal pigment (vitamin-A aldehyde) as a chromophore. By taking advantages of its photoreactivity, structure-function relationship of rhodopsins has been widely investigated with a variety of methods. Of note, recent rhodopsin research provided unexpected information regarding (i) wide distribution in various organisms, (ii) rich functional diversity and (iii) great potential for optogenetics. Here we review these three topics with future directions.

  • 西村 千秋
    2020 年 60 巻 4 号 p. 215-221
    発行日: 2020年
    公開日: 2020/07/29
    ジャーナル フリー

    Study on transient folding intermediates is one of challenging issues in protein folding. In addition to the use of fluorescence and circular dichroism combined with rapid mixing apparatus for kinetic experiments, quench-flow experiments followed by monitoring amide-proton exchange and chemical modification were used for atomic level analyses of apomyoglobin. The topics for this review include 1) possible order of the folding of helices, 2) non-native and native-like structures in the folding intermediate, 3) mutations that stabilized or destabilized intermediates, and 4) mechanism of the switch between intermediate and native structures regulated by H24 and H119 interaction.

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