Abstract
Totally in vitro protein evolution systems are powerful tools to explore the protein sequence space widely, uniformly, and rapidly. The systems require "genotype-phenotype linkage" that has been accomplished so far in three methods; ribosome display, in vitro virus display, and STABLE display systems; by physically linking protein and its gene(mRNA or DNA). Useful applications are;(i)evolutionary design of novel and improved proteins,(ii)experimental simulation of protein evolution, and(iii)high-throughput screening of protein-protein interactions for functional proteomics.
