生物物理
Online ISSN : 1347-4219
Print ISSN : 0582-4052
解説
ヒトゲノムDNAの不規則で柔軟な収納原理
前島 一博城地 保昌西野 吉則高田 英昭鎌田 福美日原 さえら
著者情報
ジャーナル フリー

2013 年 53 巻 1 号 p. 004-010

詳細
抄録

How is 2-m-long genomic DNA organized into a mitotic chromosome or nucleus? The nucleosome fiber has long been assumed to be folded into a 30-nm chromatin fiber, and further helically folded larger fiber. However, when we observed frozen hydrated human mitotic cells using cryoelectron microscopy (cryo-EM), no higher-order structures including 30-nm chromatin fibers were found. To further investigate the bulk structure of mitotic chromosomes, we performed small-angle X-ray scattering (SAXS) at SPring-8. No structural feature larger than 11 nm was detected, even on a chromosome-diameter scale (~1 μm). We also found a similar scattering pattern in interphase nuclei of HeLa cells in the range up to ~275 nm. Our findings suggest a common structural feature in interphase and mitotic chromatins: compact and irregular folding of nucleosome fibers occurs without a 30-nm chromatin structure.

著者関連情報
© 2013 by THE BIOPHYSICAL SOCIETY OF JAPAN
前の記事 次の記事
feedback
Top