Various Application of Fluorescent and Chemiluminescent Proteins

Abstract

After the gene cloning and development of GFP color variants about 20 years ago, fluorescent and luminescent proteins have become indispensable tools for biological research. Their genetically encodablity and light-emitting property has revolutionized our research ability by allowing the visualization of variety of living specimen ranging from biochemical events, proteins, cells, and organisms. Detailed understanding of the physicochemical mechanisms responsible for light generation has helped drive performance improvements and application development. Here we will cover basics of light-emitting proteins, as well as the use of them for bioimaging and biomanipulation.