Membrane Traffic in the Golgi Apparatus

Abstract

The Golgi apparatus plays a pivotal role in membrane traffic of eukaryotic cells. Cargo proteins synthesized in the endoplasmic reticulum (ER) are transported to the cis-face of the Golgi, processed and glycosylated, and then delivered from the trans-face of the Golgi for their final destinations. Debates exist, though, on the mechanism how proteins are transported through the Golgi apparatus. Here, we review current understanding of protein transport through the Golgi apparatus, and describe our models based on the observation of their dynamics by the 3D live imaging microscopy we developed.