Artificial cell membranes (giant lipid vesicles and giant liposomes) have been used for investigating lipid-biomolecules (peptides and proteins) interactions, membrane dynamics, and synthesis of proteins in simplified and isolated in vitro environments. Giant vesicles generated by conventional vesicle formation methods have limitations such as monodisperse generation, encapsulation efficiency, and asymmetric lipid membrane formation. The use of microfabrication technology facilitates the generations of well-controlled giant vesicles. In this review, we introduce the method and application of the giant vesicles using microfluidic technologies.