総説
PET分解酵素Cut190の構造基盤と高機能化
キーワード:
Ca2+ binding,
crystal structure,
cutinase,
PET depolymerization,
protein engineering,
thermal stability
2020 年 60 巻 6 号 p. 342-345
詳細
2020 年 60 巻 6 号 p. 342-345
A cutinase-like enzyme from a thermophilic isolate, Saccharomonospora viridis AHK190, Cut190, has the ability to depolymerize polyethylene terephthalate (PET). The catalytic activity and thermal stability of Cut190 are increased by Ca2+ binding. The structural analysis of Cut190 mutants in complex with metal ions and substrates elucidated the reaction mechanism regulated by Ca2+. The metal ion-binding properties, analyzed using isothermal titration calorimetry were correlated with the effects on Cut190 activity and stability, which could be improved using protein engineering. The Cut190 mutant will be used for PET chemical recycling.
