ヘモグロビンのリガンド結合とサブユニット間相互作用

抄録

The Preutz model for the cooperative ligand binding and the interactions between hemoglobin subunits is briefly described. Several results are presented indicating that the binding of the ligand or changes of spin states of the irons at some hemes of a tetramer alter the states of the other hemes as detected by spectrophotometry. ESR and NMR, which support the Perutz model. The importance of describing the difference in the states of deoxygenated hemes between totally deoxygenated Hb and the intermediates in ligand binding is stressed. Kinetic results suggestingsting the presence of the intermediate states and the kinetic parameters for half liganded tetramers are introduced.