バクテリオファージλの細胞内形成過程

電子顕微鏡観察によるその視覚化の試み

抄録

A new method was developed for the visualization of the intracellular development of phage lambda. By this new method, the presence of a complex consisting of precursor head and long DNA molecule (concatemer) can be clealy demonstrated in the host cells after infection of wild type phage. Observations of various mutants defective for head morphogenesis show that several head-related structures (empty precursor heads, head partially filled with DNA, and monsters) are present in the complex with DNA. The present investigation supports the continuous packaging of DNA into precursor heads proposed by Emmons (1974).