Examination on animal model of exercise-induced hyperuricemia with potassium-oxonate

Abstract

The purpose of this study is to examine the appropriateness which use animal model of rats for the study exercise induced-hyperuricemia, whose uricase activities were inhibited by potassium-oxonate (K-oxonate). The changes of purine metabolites in blood and skeletal muscle from rats following the exhaustive running exercise were observed. Rats were divided into three groups: resting control groups treated with K-oxonate (n=28) and without K-oxonate treatment (n=5), and an exercised group treated with K-oxonate (n=48). Purine metabolites were assayed by HPLC at 0,30 min, 1, 2, 3, and 5 hours after exhaustive exercise. Plasma allantoin concentration was suppressed by K-oxonate in both the exercise and control groups. There was a significant increase in plasma inosine concentrations from 1 hour to 5 hour of recovery. Plasma hypoxanthine was slightly increased immediately after exercise. In extensor digitorum longus muscle, ATP levels decreased, but IMP levels increased immediately after exercise. Inosine did not show a significant change, hypoxanthine levels were slightly increased following exercise in extensor digitorum longus muscle. The ATP levels in soleus muscle showed a tendency to decrease compaerd with the controls immediately after exercise, but it was not significant change. There were not significant changes in other purine metabolites in soleus muscle throughout experiment period. These results suggest that the use of K-oxonate treated rat model is appropriate for the study of the mechanism of exercise induced-hyperuricemia.