Mycorrhizal Fungi Isolated from Liparis krameri (Orchidaceae) in Japan

抄録

Mycorrhizal fungi of Liparis krameri from eight localities covering most of its distribution area in Japan were analyzed. Most of the fungi isolated from L. krameri were identified as Tulasnella spp. (Tulasnellaceae) based on ITS sequences. Tulasnella isolates were obtained from all localities. The results suggested that Tulasnella was the main mycorrhizal partner of L. krameri. Molecular phylogeny showed that the Tulasnella partners were located in a specific clade in the genus, suggesting that L. krameri has high specificity for its mycorrhizal partner. The mycorrhizal partners of epiphytic L. truncata were different from those of terrestrial L. krameri despite belonging to the same clade. It is likely that mycorrhizal partners changed during their life-form evolution.