Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Mechanism of Superoxide Generation System in Indomethacin-Induced Gastric Mucosal Injury in Rats
Junko TANAKAYasukatu YUDATosio YAMAKAWA
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2001 Volume 24 Issue 2 Pages 155-158

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Abstract

We studied the mechanism of the superoxide generation system in indomethacin-induced gastric mucosal injury. First, 10 mM indomethacin had no direct effect on xanthine oxidase (XOD) activity. Next, we found that NADPH oxidase activity in polymorphonuclear leukocytes (PMN) of peripheral blood was significantly increased 6 h after administration of indomethacin. This phenomenon was inhibited by the injection of the NADPH oxidase inhibitor, diphenylene iodonium chloride (DIC). Activation of NADPH oxidase caused the component, p47phox to be translocated to the plasma membrane. Since indomethacin did not directly activate NADPH oxidase, we sought another route of activation of PMN. As IL-1 and TNF α play in the inflammation, we examined these cytokines in this study. TNF α was not detected but IL-1 was increased significantly 30 min after administration of indomethacin.

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© 2001 The Pharmaceutical Society of Japan
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