Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Nitric Oxide Donor Sodium Nitroprusside Induces Neurotoxicity in Cerebellar Granular Cell Culture in Rats by an Independent Mechanism from L-Type or Dantrolene-Sensitive Calcium Channels
Akçahan GepdiremenAhmet HacimüftüogluMehmet Emin BüyükokurogluHalis Süleyman
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2002 Volume 25 Issue 10 Pages 1295-1297

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Abstract

The effects of sodium nitroprusside (SNP) in rat cerebellar granular cell culture were investigated in the present study. All doses of the SNP (10, 25, 50, 100, 250, 500 μM) were able to induce cell death compared with control values (p<0.001 for all groups tested). Interestingly enough, a nonlinear dose–response curve was obtained for SNP-induced neurotoxicity. We also investigated the possible neuroprotective effects of nimodipine and dantrolene, alone or in combination. Both drugs failed to prevent neuronal cell death at the doses tested, either alone or in combination. Despite the fact that the most effective dose was a dantrolene concentration of 10 μM with SNP 500 μM and a concentration of 1 μM with SNP 50 μM, the differences were insignificant statistically. According to our results, SNP-induced cerebellar toxicity appears to be an independent reaction from L-type or endoplasmic reticulum calcium currents.

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© 2002 The Pharmaceutical Society of Japan
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