Application of Green Fluorescent Protein to Affinity Electrophoresis; Affinity of IgG-Binding Domain C from Streptococcal Protein G to Mouse IgG1

Abstract

Affinity electrophoresis (AEP) using green fluorescent protein (GFP) was studied. We constructed a fusion protein that linked S147PGFP and IgG binding domain C from streptococcal protein G (GFP–SpGC). The affinity of GFP–SpGC for mouse IgG1 was measured. The AEP using GFP does not require a staining step after electrophoresis, and was successful with a non-purified sample. Therefore, this method is simple and useful for measuring many samples such as those used in mutational studies.