J-STAGE Home  >  Publications - Top  > Bibliographic Information

Biological and Pharmaceutical Bulletin
Vol. 25 (2002) No. 8 P 1018-1021

Language:

http://doi.org/10.1248/bpb.25.1018

Regular Articles

Lumin was orally administered to mice daily for 3 d, and on the day following the final administration, mice were sacrificed and splenocytes were stimulated with lipopolysaccharide (LPS). Splenocytes obtained from lumin-treated mice showed enhanced production of interferon γ (IFN-γ) and increased percentages of CD3+ cells. Although T cells are considered to be the source of IFN-γ, it is unlikely that LPS directly stimulates T cells. Next we performed neutralization experiments using a monoclonal antibody (mAb) against interleukin (IL-)12 because this cytokine, which is produced by macrophages, has the direct ability to induce IFN-γ production and the proliferation of activated T cells. This antibody inhibited IFN-γ production by splenocytes. We thus show that orally administered lumin enhances IFN-γ production by splenocytes when the latter are stimulated with LPS, a phenomenon that was observed in correlation with activation of T cells by IL-12, that is produced by macrophages.

Copyright © 2002 The Pharmaceutical Society of Japan

Article Tools

Share this Article