Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Effect of the Water-Soluble and Non-dialyzable Fraction Isolated from Senso (Chan Su) on Lymphocyte Proliferation and Natural Killer Activity in C3H Mice
Yasuharu ShimizuEiji InoueChihiro Ito
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2004 Volume 27 Issue 2 Pages 256-260

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Abstract

We found lymphocyte proliferating substances in the water-soluble and non-dialyzable fraction prepared from the crude drug Senso (Chan Su). The effect of this fraction was increased by affinity chromatography using the concanavalin A–agarose. By analyzing the fraction using sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and lectin blotting, we estimated that one of the active substances of this fraction is a glycoprotein that has about 13 kDa of molecular weight and D-mannose within the molecule. The purified fraction increased the IL-2 and the IL-12 level in the supernatant of spleen cell culture, and increased the natural killer activity of spleen lymphocyte in C3H/HeN mice. These results show that Senso contains immunopotentiating substances that may serve as an immunomodulator in an organism.

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© 2004 The Pharmaceutical Society of Japan
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