Abstract
We attempted to develop an experimental system for evaluation of stress effect on ethyl alcohol metabolism by the radiorespirometric analysis using [14C]ethyl alcohol. Each rat was immobilized in an adjustable restraint device for 3 h. The excretion of 14CO2 in the breath was significantly decreased by restraint stress. The liver alcohol dehydrogenase activity was also decreased by restraint stress. We think that radiorespirometric analysis may be useful for the evaluation of stress effect on real metabolism of ethyl alcohol in the liver.
