Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
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Increase of Caspase-3 Activity by Lignans from Machilus thunbergii in HL-60 Cells
Bo-Young ParkByung-Sun MinOk-Kyoung KwonSei-Ryang OhKyung-Seop AhnTae-Jin KimDoo-Young KimKiWhan BaeHyeong-Kyu Lee
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2004 Volume 27 Issue 8 Pages 1305-1307

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Abstract

Nine lignans and two butanolides were isolated from the stem bark of Machilus thunbergii and their structures were identified as machilin A (1), licarin B (2), zuonin B (3), macelignan (4), secoisolancifolide (5), isolancifolide (6), oleiferin C (7), meso-dihydroguaiaretic acid (8), licarin A (9), machilin F (10), and nectandrin B (11) by spectroscopic means. These compounds were assessed for their abilities to activate a caspase-3 activity in human promyeloid leukemic HL-60 cells. The intracellular caspase-3 activity of macelignan (4), oleiferin C (7), meso-dihydroguaiaretic acid (8), and licarin A (9) increased approximately 3.04, 6.16, 2.10, and 3.10-fold at 100 μM over that of untreated control. In addition, compounds 4, 7, 8, and 9 induced internucleosomal DNA fragmentation in HL-60 cells.

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© 2004 The Pharmaceutical Society of Japan
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